Composite

Part:BBa_K2022008

Designed by: Gavin Sutton   Group: iGEM16_UNSW_Australia   (2016-10-13)

T7 Expression Construct for tolR, Periplasmic Domain

This part contains tolR, Periplasmic Domain (BBa_K2022002) under the control of a strong T7 promoter and RBS (both from BBa_K525998). Also included is double terminator (BBa_B0017).

Usage and Biology

In DE3 strains of E. coli, this construct is effectively IPTG-inducible, enabling easy control of expression

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None